What is a Nano-Trap?

Camelids such as camels, llamas, and alpacas possess an immune repertoire of three subclass IgG antibodies: IgG1, IgG2, and IgG3. IgG1 is a conventional IgG composed of two heavy chains and two light chains. IgG2 and IgG3 are heavy-chain-only IgG antibodies (HCAbs) that can be distinguished by their hinge regions. These HCAbs lack the CH1 domain of the heavy chain and are devoid of any light chain. The binding domain of a heavy-chain-only IgG is called a Nanobody or VHH. Nanobodies have excellent binding properties and can be recombinantly expressed at constant high quality with no batch-to-batch variation. Nano-Traps are Nanobodies conjugated to beads and are ready-to-use. They enable IP performance superior to that of conventional IgG antibodies.

Benefits: • No heavy & light antibody chains • Stringent washing conditions • Low host cell protein background • Very efficient pulldown, no protein in flow-through • High affinity to bind proteins expressed at low levels • Short incubation (30-60 min) • Recombinant expressed and validated

Available targets: 

... and more!

GFP-TRAP:

Ready-To-Use Beads for fast and efficient immunoprecipitation

The GFP-Trap® belongs to ChromoTek’s Nano-Trap family of ready-to-use pull-down reagents. GFP-Trap® 
is the benchmarking reagent for one-step immunoprecipitation (IP) of GFP-fusion proteins. The GFP-Trap® 
consists of an anti-GFP Nanobody coupled to beads. The ChromoTek GFP-Trap® provides a higher level of 
immunoprecipitation performance than conventional anti-GFP antibodies. Next to immunoprecipitation, GFP
Trap® can be applied in Co-IP, Co-IP/-mass spectrometry, on-bead assays, and ChIP/RIP analysis.

Low background, no extra bands & high specificity will improve your pulldown assay significantly.

Most cited monoclonal anti-GFP antibody!

Ready-to-use GFP-Trap formats

The GFP-Trap® consists of an anti-GFP Nanobody conjugated to different resins. The GFP-Trap® is  available as Agarose, Magnetic Agarose, Magnetic Particles M-270, or 96-well plates:  • GFP-Trap® Agarose for the lowest background and  high binding capacity IP; • GFP-Trap® Magnetic Agarose for magnetic separation and high binding capacity IP; • GFP-Trap® Magnetic Particles M-270 for pull-down  of large proteins/complexes; • GFP-Trap® Multiwell Plates for high-throughput applications and ELISA; • GFP-Trap® kits that include lysis buffer for mammalian  cells, wash, and elution buffer

Immunoprecipation of large proteins/protein complexes

GFP-Trap® Magnetic Particles M-270 are recommended for the investigation of large proteins, protein complexes, or multimers (> 200 kDa). Magnetic Particles M-270 are solid beads, whereas Magnetic  Agarose and Agarose are porous beads; large GFP fusion proteins, multimers, or bulky complexes with binding partners may be too large to diffuse into the pores of those agarose-based beads and, therefore, cannot be bound effectively.  Please note, 200 kDa is an approximate value based on empirical studies. The cut-off can also depend on the protein shape.

All Nano-Traps

... check here for all the list!